RNA Preparation and Purification
Biochain Institute Inc Total RNA - Monkey (Cynomolgus) Normal Tissue: Diaphragm, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Ductus Deferens, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Research Products International Corp ZR-96 RNA Clean & Concentrator Kit, 2 x 96 Preps
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
Biochain Institute Inc Total RNA - Human Tumor Tissue: Bone, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA poly
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Submaxillary gland, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Thymus, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Mouse Normal Tissue: Small Intestine, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
New England Biolabs, Inc. NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina -24 rxns – 24 reactions
The NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina uses a template switching method to generate full length cDNAs directly from single cells or 2 pg - 200 ng RNA, followed by conversion to sequence-ready libraries using the Ultra II FS workflow. This unique workflow enables generation of the highest yields from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance. Please note that adaptors and primers are not included in the kit and are available separately.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Lung: Right Upper Lobe, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zyagen Labs Mouse C57 bone marrow RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse BONE MARROW tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 96 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Skin, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zymo Research Corporation Direct-zol™ RNA PreWash (Concentrate) (40 ml)
Direct-zol RNA Pre-wash (40 ml) for use with Direct-zol RNA Purification Kits for rapid isolation of high-quality total RNA including small RNAs (>17 nt) from samples in TRI-Reagent, TRIzol or similar.
Zymo Research Corporation Quick-RNA™ MiniPrep Kit (200 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The procedure of Quick-RNA™ Miniprep Kit uses unique spin-column technology that results in highquality total RNA (including small/microRNAs) and is ready for Next-Gen Sequencing, RT/qPCR, hybridization, etcThe Quick-RNA™ Kits yields high quality total RNA.High levels of genomic DNA contamination arepresent in the preps from Suppliers Q & P but not withthe Quick-RNA™ Kits. Total RNA was isolated fromhuman epithelial cells.RNA isolated with the Quick-RNA™ Kits is DNA-free.Samples isolated with Supplier Q's kit are provided forcomparison. Total RNA was isolated from 106 humanepithelial cells (with in-column DNase treatments forboth kits). Each amplification curve represents anaverage of three independent isolation experiments.
Zyagen Labs Rat bone marrow total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy Sprague Dawley RAT BONE MARROW tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.